The CFX Opus System is the next evolution in qPCR from Bio-Rad. With improved thermal performance and optical shuttle system, your data will be more consistent than ever. A sleek, modern design includes a refreshed and easy-to-use interface with new connectivity options and features.

Key Benefits

• More uniform thermal performance.
• Expanded connectivity — Wi-Fi, ethernet, and USB.
• Network storage drive access for excellent data management.
• Shuttle optical system continues to yield consistent optical measurements across your sample plate.

The kit can be used for up to 94 samples and can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs.

The protocol has been validated by several certification bodies and provides results in much less time.

Bio-Rad offers a complete solution with standardized reagents, enrichment media, PCR kits, confirmation agars, and PCR detection systems, as well as software for automated analysis specific to each test.

The kit components include lysis reagent for DNA extraction, fluorescent probes, PCR amplification mix, positive and negative PCR controls.

The kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

It can be used for up to 94 samples and can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. The iQ-Check Salmonella II protocol has been validated by several certification bodies and provides results in much less time.

Bio-Rad offers a complete solution with standardized reagents, enrichment media, PCR kits, confirmation agars, and PCR detection systems, as well as software for automated analysis specific to each test.

Color indicators provide a high level of contrast and accurate reading of Enterobacteriaceae colonies, making them easy to count using an automated colony counter.

Key Benefits

• Quick response: complete

  results in 24 hr

• Easy to read: red colonies with high level of contrast
• Cost effective: only one plate required per sample
• No confirmation required
• Certified NF VALIDATION according to the ISO 16140 standard

Key Benefits

• Fast results

Less than 42 hr to detect Salmonella spp. when using the short protocol

Fast confirmation (less than 1 minute)

• 1 broth and 1 plate

Saves money

Saves handling time

• Low false positive rate (<0.3% for all matrices)

Saves on unnecessary confirmations

Detection is based on a specific enzymatic activity common to all species of Cronobacter. Hydrolysis of the chromogenic substrate 5-bromo-4-chloro-3-indol α-D-glucopyranoside leads to the formation of typical turquoise-colored colonies.

The medium includes compounds carefully selected to promote the growth of Cronobacter spp. while suppressing interfering flora, giving the medium increased selectivity. Competing bacteria, if not completely inhibited, will form light purple or gray colonies.

Key Benefits:

• Easy-to-read chromogenic reaction Blue to blue-green colonies
• Cost-effective short protocol
• No selective enrichment in mLST + Vancomycin
• Negative results in less than 42 hr
• Validation
• Certified NF VALIDATION according to the ISO 16140:2003 standard for the detection of Cronobacterspp. in infant milk powders

Due to its unique sensitivity, it enables food microbiology laboratories to meet regulatory requirement for this organism.

The sensitivity of the medium has been specially optimized to allow growth of all pathogenic B. cereus strains, even those that are difficult to culture, such as B. cytotoxicus. The interpretation of RAPID’B.cereus plates is further facilitated by the high level of contrast between the color of the colonies and the agar.

Key Benefits

• Quick response: complete results in 24 +/- 3 hr
• Easy-to-read, red colonies with high level of contrast: combining strong coloration of typical colonies and the contrast of the halo, accurate counting is very easy to perform
• Clear enumeration of the sensitive B. cytotoxicus
• Inhibition of background flora on raw matrices
• No confirmation required

RAPID’ L.mono is the only chromogenic method to be validated for L. monocytogenes and Listeria spp. detection on the same plate.

Key Benefits:

• Listeria spp. detection 24 and 48 hr after enrichment
• L. monocytogenes enumeration in 24 hr without confirmation
• L. monocytogenes confirmation in 24 hr after using the iQ-Check Listeria monocytogenes II Kit
• Simple and cost effective
• Only 1 broth, and 1 plate required for each sample
• Easy confirmation
• Easy-to-Read Chromogenic reaction
• L. monocytogenes (PIPLC+/xylose-) forms blue colonies
• L. ivanovii (PIPLC+/xylose+) forms blue-green colonies with distinct yellow halos
• Other Listeria species form white colonies

Key Benefits:

• Simultaneous enumeration of E. coli and coliforms at 37°C without further confirmation

• Specific enumeration of E. coli at 44°C without further confirmation
• Results in 18–24 hr
• Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Principle

The medium is based on the simultaneous detection of β-D-Glucuronidase (GLUC) and β-D-Galactosidase (GAL) activities. E. coli (GAL+/GLUC+) forms violet to pink colonies, while other coliforms (GAL+/GLUC-) form blue to green colonies.

Detection of GLUC confers a high specificity to the method because E. coli is one of the few species of Enterobacteriaceae to possess this enzyme. Growth of interfering flora is inhibited, especially at 44°C, conferring high specificity to the medium.

Key Benefits:

• High specificity to E. coli O157 serotype H7

• Only 1 plate required per sample
• Results in 48 hr
• Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Simple Protocol

RAPID’E.coli O157:H7 Medium provides laboratories with a rapid and cost-effective solution for E. coli O157:H7 detection, given that a single plate is required for each sample. The latex test for confirmation has been validated for use on isolated colonies grown on RAPID’E.coli plates.

Other Applications of RAPID’E.coli O157:H7 Medium

• As a second selective medium in different standardized protocols (for example, the ISO 16654 reference method)
• To confirm positive results obtained with the iQ-Check E. coli O157:H7 PCR Detection Kit

Easy-to-Read Results

RAPID’E.coli O157:H7 medium permits the detection of E. coli O157 serotype H7 using specific chromogenic substrates and biochemical activities.

• Typical E. coli O157:H7 (Sorbitol/SORB +, β-Glucuronidase/GLUC +) colonies are characteristically convex, 1–2 mm in diameter, and dark blue to black in color
• Atypical GLUC + E. coli O157:H7 are detected and present as dark blue to black colonies
• Atypical SORB + E. coli O157:H7 are detected and present as blue to turquoise blue colonies

RAPID’E.coli O157:H7 Medium is very specific. Most other bacteria and yeasts are inhibited or produce a different color. The selectivity of RAPID’E.coli O157:H7 Medium can be improved by adding selective agents such as novobiocin and potassium tellurite.