IHC-P: IHC analysis of paraffin-embedded human small cell lung carcinoma using #79602

Mediator of Cell Adhesion and Signal Transduction

Integrin beta-5 is a receptor that primarily forms the complex αVβ5 with its α subunit and binds to extracellular matrix components such as vitronectin. It plays a crucial role in regulating cell adhesion, migration, proliferation, angiogenesis, and phagocytosis. Depending on the extracel lular environment, it is known to activate FAK and MAPK/ERK pathways, promoting cell survival and proliferation, as well as sensing intracellular signals and altering interactions with the extracellular environment. Integrin αVβ5 plays an important role in growth factor-induced tumor angiogenesis, and it has been reported that coordinated signal transduction between Integrin αVβ5 and growth factors controls endothelial cell proliferation and survival. #79602 is suitable for IHC-P and IF-IC using human samples. For IF-F and IF-IC using mouse samples, #27874 is suitable

IFF-F: Confocal IF analysis of fixed frozen brain from an amyloid mouse model of Alzheimer’s disease labeled with #77438 (red), Iba1/AIF-1 (E4O4W) Feline Chimeric Monoclonal Antibody #87276 (green), TREM2 (E7O7Q) Rabbit Monoclonal Antibody #89956 (gray), and ProLong Gold Antifade Reagent with DAPI
#8961 (blue). Rabbit primary antibody is detected using an anti-rabbit Fc-specific secondary antibody

Proteins that accumulate in the brain in Alzheimer’s disease

Amyloid β (Aβ) is a peptide that is cleaved from APP and aggregates and accumulates in the brain. The main variants are Aβ1-42, which has high aggregation properties and forms the nuclei of senile plaques from
the early stages of the disease, and Aβ1-40, which is characterized by deposition in the blood vessel walls (CAA: cerebral amyloid angiopathy). #77438, 51867, and 99675, which can specifically detect Aβ1-40, are chimeric antibodies in which only the Fc region has been rearranged to horse, cat, and mouse. Simultaneously, multiplex immunohistochemistry using antibodies for different host markers such as nerves and neuroin flammation allows for accurate evaluation of the spatial relationships around lesions. #77438, 51867, and 99675 are suitable for IF-F using human samples, and reactivity in mouse, rat, monkey, and bovine samples is predicted based on the homology of the recognized amino acid sequences.

• Get precise results with Q5’s high fidelity (~280x Taq)
• Reduce turnaround time through fast cycling conditions, with extension times of 5-15 seconds/kb
• Optimized formulation enables robust, reliable amplification of diverse targets (from high AT or high GC) up to 20 kb
• Improve reaction specificity with a hot start aptamer that allows a convenient room temperature setup
• Compatible with a 62°C universal annealing temperature protocol. Learn more in our application note
• Also available: Q5 standalone polymerase (NEB #M0491, NEB #M0493, NEB #M0515) and master mix formats (NEB #M0492, NEB #M0494, NEB #M0578, NEB #M0580)