IHC-P: IHC analysis of paraffin-embedded ALS spinal cord using #58330.

High-Molecular-Weight Tau

Tau is a microtubule-associated protein (MAP) that promotes and stabilizes microtubule assembly within axons. Peripheral Tau, also referred to as “Big Tau,” is a high-molecular-weight (HMW) isoform of the Tau protein characterized by the insertion of exon 4a, which encodes approximately 250 amino acids, within the N-terminal domain. While Tau is expressed as multiple low-molecular-weight (LMW) isoforms in the majority of neurons within the central nervous system (CNS), it is expressed as the HMW isoform in peripheral nervous system (PNS) neurons and a small subset of CNS neurons. Compared to LMW Tau isoforms, peripheral Tau exhibits lower hydro phobicity and lower predicted β-sheet content, making it less prone to aggregation. #58330 is suitable for WB, IP, and IHC-P) with human samples. #70448, which detects brain-derived Tau, is validated for WB using human samples.

IF-F: Confocal IF analysis of fixed frozen mouse testis using #79314 and beta3-Tubulin (E9F3E) Mouse Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #82087 (magenta pseudocolor). Free secondary binding sites were then blocked with Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 prior to co-labeling with alpha-Smooth Muscle Actin (D4K9N) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate) #60839 (red) and ProLong Gold Antifade Reagent with DAPI #8961 (blue).

A Master Regulator of EMT

ZEB2 (also known as SIP1) is a master regulator of epithelial-mesenchymal transition (EMT) that represses the transcription of key targets, including E-cadherin, via binding to E-box sequences. It interacts with Smad complexes and associated cofactors downstream of TGF-β/BMP signaling to spatiotemporally regulate neural crest cell migration and neural stem cell fate determination during development. In humans, haploinsufficiency of ZEB2 is well established as the underlying cause of Mowat-Wilson syndrome. Furthermore, ZEB2 has been reported to participate in a diverse array of biological phenomena; these include cancer invasion and metastasis, chemoresistance, immune evasion mechanisms mediated by the induction of PD-L1/CCL2 expression in the tumor microenvironment, T/NK cell differentiation, phenotypic maintenance of myeloid cells, the pathogenesis of hematological malignancies, and organ fibrosis associated with chronic inflammation. #79314 is validated for WB, IP, IF-F, and IF-IC using human, mouse, rat, and monkey samples.