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Air-liquid interface culture: Building physiologically relevant airway models
Traditional cell culture systems often fall short of replicating the complex environment of the human airway, limiting their predictive power in respiratory research. To address these limitations, researchers increasingly rely on physiologically relevant in vitro models that better reflect human airway biology. Among these, air-liquid interface (ALI) culture bridges the gap between simple two-dimensional systems and complex animal models, offering a human-based approach for studying respiratory function and disease in vitro.
New Products this month from CST!
NG2/CSPG4 (F8X3M) Rabbit
Monoclonal Antibody #54851
IF-F: Confocal IF analysis of fixed mouse cerebellum labeled with #54851 (green), TMEM119 (E4B9S) Mouse Monoclonal Antibody #98778 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Proteoglycan Involved in Cell Scaffolding and Signaling
NG2 is a transmembrane chondroitin sulfate proteoglycan present on the cell surface. It promotes cell proliferation, migration, and metastasis through phosphorylation by PKCα and ERK. It is also expressed in vascular pericytes to regulate angiogenesis, and in the nervous system, it is involved in myelin repair as a marker of oligodendrocyte precursor cells. It is highly expressed in intractable cancers such as melanoma, glioblastoma, and triple-negative breast cancer, and is associated with treatment resistance and metastasis. Taking advantage of its unique expression pattern, NG2 is currently being clinically applied as a promising target for cancer immunotherapy, such as CAR-T cell therapy. #54851 is suitable for WB, IP, IF-F, IF-IC, FC-FP, and FC-L analysis using human, mouse, and rat samples.
BRD9 (F6F1S) Rabbit Monoclonal
Antibody #98202
ChIP: ChIP were performed with cross-linked chromatin from NIH/3T3 cells and either #98202 or Normal Rabbit IgG #2729, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human RCN3 promoter primers, human ZFP948 intron 1 primers, and SimpleChIP® Mouse Intracisternal A-Particle (IAP) LTR Primers #85916. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin Remodeling Component
BRD9, a component of the non-canonical BAF complex, recognizes acetylated histones via its bromodomain and targets the complex to specific gene promoters and enhancers. BRD9 is particularly involved in maintaining stem cell pluripotency, cell differentiation, and regulating immune responses, regulating the expression of genes essential for cell fate determination. It has also been reported to be essential for tumor survival in synovial sarcoma and malignant rhabdoid tumors, which possess characteristic gene fusions. BRD9 is also involved in the proliferation of acute myeloid leukemia (AML), and in recent years, development of drugs targeting BRD9, such as PROTACs (targeted protein degradation inducers), has been progressing. #98202 is suitable for WB, IP, and ChIP using mouse and rat samples.
New Products This Month from NEB!
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